Data/hora: 10/06/2024 06:22:16

Provedor de dados: | 52
País: | Brazil
Título: | Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca
Autores: | Morais,Glaucia Almeida de; Takaki,Massanori.
Data: | 1998-06-01
Ano: | 1998
Palavras-chave: | Α-amylase; Β-amylase; Determination; Selective inactivation; Phaseolus vulgaris.
Resumo: | Determination of α- and β-amylase activity in the extracts of cotyledons of Phaseolus vulgaris L. cv. cariocawas done using selective inactivation of α-amylase by lowering the pH of the incubation medium or by the use of EDTA as inhibitor or selective inactivation of β-amylase by the use of HgCl2 or by heating to 70ºC in the presence of CaCl2; and still by using the reagent starch azure for specific determination of α-amylase. Results indicated that the methods used were inappropriate in this case, being indicated the determination of total amylase activity.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100003
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89131998000100003
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.41 n.1 1998
Fonte: | info:eu-repo/semantics/openAccess