Data/hora: 08/06/2024 01:13:03

Provedor de dados: | Brazilian Archives of Biology and Technology
País: | Brazil
Título: | In Vitro Mid-Term Conservation of Acorus calamus L. via Cold Storage of Encapsulated Microrhizome
Autores: | Quraishi,Afaque; Mehar,Snigdha; Sahu,Durga; Jadhav,Shailesh Kumar.
Data: | 2017-01-01
Ano: | 2017
Palavras-chave: | Aromatic plant; Encapsulation; Sweet Flag; Synthetic seeds; Plant tissue culture.
Resumo: | ABSTRACT In vitro rhizome production, encapsulation and cold storage of Acorus calamus were attempted for its propagation and ‘true-to-type’ conservation. Shoot cultures were initiated using underground rhizome buds, on 6-benzyladenine (BA) containing Murashige and Skoog (MS) medium. Maximum microrhizome production was observed in presence of 33.3 µM BA, on modified MS medium containing 6% sucrose, 100 mg/L citric acid and 1 g/L polyvinyl pyrrolidone-40. Synthetic seeds were produced from regenerated microtubers by encapsulation in calcium alginate beads. These synthetic seeds were stored in complete darkness at 100C temperature for different durations for mid-term conservation. After cold storage, synthetic seeds were re-cultured in vitro, 100% survival was recorded after the storage of 1, 3 or 6 months; and 80% survival was observed after the storage of 12 months. The microrhizomes were produced roots in 4.9 µM indole-3-butyric acid containing half strength MS medium. All the regenerated plantlets were successfully transferred to field after acclimatization. It is the first report on successful one year in vitro cold storage of A. calamus.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100412
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/1678-4324-2017160378
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.60 2017
Fonte: | info:eu-repo/semantics/openAccess