Data/hora: 06/06/2024 04:31:49

Provedor de dados: 	Brazilian Archives of Biology and Technology	
País: 	Brazil	
Título: 	Hydrolysis of galacto-oligosaccharides in soy molasses by α -galactosidases and invertase from Aspergillus terreus	
Autores: 	Reis,Angélica Pataro; Guimarães,Valéria Monteze; Ferreira,Joana Gasperazzo; Queiroz,José Humberto de; Oliveira,Maria Goreti Almeida; Falkoski,Daniel Luciano; Almeida,Maíra Nicolau de; Rezende,Sebastião Tavares de.	
Data: 	2010-06-01	
Ano: 	2010	
Palavras-chave: 	Aspergillus terreus; Galactooligosaccharides; Α-galactosidase; Invertase; Soy molasses.	
Resumo: 	Two α -galactosidase (P1 and P2) and one invertase present in the culture of Aspergillus terreus grown on wheat straw for 168 h at 28ÂºC were partially purified by gel filtration and hydrophobic interaction chromatographies. Optimum pH and temperatures for P1, P2 and invertase preparations were 4.5-5.0, 5.5 and 4.0 and 60, 55 and 65ÂºC, respectively. The K M app for Ï -nitrophenyl-α -D-galactopyranoside were 1.32 mM and 0.72 mM for P1 and P2, respectively, while the K M app value for invertase, using sacarose as a substrate was 15.66 mM. Enzyme preparations P1 and P2 maintained their activities after pre-incubation for 3 h at 50ÂºC and invertase maintained about 90% after 6 h at 55 ÂºC. P1 and P2 presented different inhibition sensitivities by Ag+, D-galactose, and SDS. All enzyme preparations hydrolyzed galacto-ologosaccharides present in soymolasses.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300029	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132010000300029	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.53 n.3 2010	
Fonte: 	info:eu-repo/semantics/openAccess