Data/hora: 05/06/2024 04:46:58

Provedor de dados: | 52
País: | Brazil
Título: | Hydrolysis of galacto-oligosaccharides in soy molasses by α -galactosidases and invertase from Aspergillus terreus
Autores: | Reis,Angélica Pataro; Guimarães,Valéria Monteze; Ferreira,Joana Gasperazzo; Queiroz,José Humberto de; Oliveira,Maria Goreti Almeida; Falkoski,Daniel Luciano; Almeida,Maíra Nicolau de; Rezende,Sebastião Tavares de.
Data: | 2010-06-01
Ano: | 2010
Palavras-chave: | Aspergillus terreus; Galactooligosaccharides; Α-galactosidase; Invertase; Soy molasses.
Resumo: | Two α -galactosidase (P1 and P2) and one invertase present in the culture of Aspergillus terreus grown on wheat straw for 168 h at 28ÂºC were partially purified by gel filtration and hydrophobic interaction chromatographies. Optimum pH and temperatures for P1, P2 and invertase preparations were 4.5-5.0, 5.5 and 4.0 and 60, 55 and 65ÂºC, respectively. The K M app for Ï -nitrophenyl-α -D-galactopyranoside were 1.32 mM and 0.72 mM for P1 and P2, respectively, while the K M app value for invertase, using sacarose as a substrate was 15.66 mM. Enzyme preparations P1 and P2 maintained their activities after pre-incubation for 3 h at 50ÂºC and invertase maintained about 90% after 6 h at 55 ÂºC. P1 and P2 presented different inhibition sensitivities by Ag+, D-galactose, and SDS. All enzyme preparations hydrolyzed galacto-ologosaccharides present in soymolasses.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300029
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132010000300029
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.53 n.3 2010
Fonte: | info:eu-repo/semantics/openAccess