Data/hora: 09/06/2024 05:25:02

Provedor de dados: | Brazilian Archives of Biology and Technology
País: | Brazil
Título: | Bauhinia forficata link shoot regeneration: histological analysis of organogenesis pathway
Autores: | Mello,Marcia O.; Melo,Murilo; Appezzato-da-Glória,Beatriz.
Data: | 2000-01-01
Ano: | 2000
Palavras-chave: | Tissue culture; Organogenesis; Anatomy.
Resumo: | Plant regeneration was achieved from cells of callus induced from hypocotyl segments of Bauhinia forficata on half strength Murashige and Skoog culture medium supplemented with several concentrations of BAP. Within 40 days of culture shoot buds formation was observed on callus surface. Calli were then transferred to a same composition culture medium without plant growth regulator in order to induce shoot elongation. Histological studies indicated that in vitro plant regeneration in B. forficata occurred through indirect organogenesis. Meristemoids consisting of small cells with dense cytoplasm and prominent nuclei were randomly distributed throughout the callus surface indicating early stages of shoot bud differentiation. Shoots developed de novo from superficial layers of cells and the pattern of shoot origin and development were very similar to those previously described for other leguminous species.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400013
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132000000400013
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.43 n.4 2000
Fonte: | info:eu-repo/semantics/openAccess