Data/hora: 17/06/2024 15:25:38

Provedor de dados: | 52
País: | Brazil
Título: | Damage assessment of the equine sperm membranes by fluorimetric technique
Autores: | Celeghini,Eneiva Carla Carvalho; Andrade,André Furugen Cesar de; Raphael,Cláudia Fernandes; Nascimento,Juliana; Ticianelli,Janahi Souza; Arruda,Rubens Paes de.
Data: | 2010-12-01
Ano: | 2010
Palavras-chave: | Stallion; Acrosome; Plasma membrane; Mitochondria; Fluorescent probes.
Resumo: | To validate a practical technique of simultaneous evaluation of the plasma, acrosomal and mitochondrial membranes in equine spermatozoa three fluorescent probes (PI, FITC-PSA and MITO) were associated. Four ejaculates from three stallions (n=12) were diluted in TALP medium and split into 2 aliquots, 1 aliquot was flash frozen in liquid nitrogen to induce damage in cellular membranes. Three treatments were prepared with the following fixed ratios of fresh semen: flash frozen semen: 100:0 (T100), 50:50 (T50), and 0:100 (T0). A 150-µL aliquot of diluted semen of each treatment was added of 2 µL of PI, 2 µL of MITO and 80 µL of FITC-PSA; incubated at 38.5ºC/8 min, and sperm cells were evaluated by epifluorescent microscopy. Based in regression analysis, this could be an efficient and practical technique to assess damage in equine spermatozoa, as it was able to determine the sperm percentage more representative of the potential to fertilize the oocyte.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600004
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132010000600004
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.53 n.6 2010
Fonte: | info:eu-repo/semantics/openAccess