Data/hora: 16/06/2024 00:31:19

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling	
Autores: 	Cardoso,Milena Barcelos; Kaltchuk-Santos,Eliane; Mundstock,Elsa Cristina de; Bodanese-Zanettini,Maria Helena.	
Data: 	2004-09-01	
Ano: 	2004	
Palavras-chave: 	Glycine max; FDA; Binucleate pollen grains; Multinucleate pollen grains; Spontaneous diploidization.	
Resumo: 	Anthers obtained from flowers buds of soybean cultivar IAS-5 were cultured in two basal culture media (B5 and B5 long). Cytological examinations of the in vitro anthers were performed during the first 20 days of culture to assay the viability (by propionic-carmine and fluorescein diacetate tests) and the stage of development of pollen grains. The frequencies of viable pollen grains varied significantly between bud sizes on the propionic-carmine analysis. The basal culture media and bud size had no clear effect on the frequencies of binucleate symmetrical and multinucleate pollen grains. Chromosome counts of metaphasic microspores throughout the culture period showed microspores with higher ploidy level in addition to normal chromosome number (n=20).	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500005	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132004000500005	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.47 n.5 2004	
Fonte: 	info:eu-repo/semantics/openAccess