Data/hora: 11/06/2024 13:22:49

Provedor de dados: 	Brazilian Archives of Biology and Technology	
País: 	Brazil	
Título: 	Genetic variability in regenerated Metarhizium flavoviride protoplasts	
Autores: 	Kuklinsky-Sobral,Júlia; Luna-Alves-Lima,Elza Áurea de; Araújo,Janete Magali de; Azevedo,João Lúcio.	
Data: 	2004-03-01	
Ano: 	2004	
Palavras-chave: 	Metarhizium flavoviride; Protoplasts; Genetic variability; Benomyl; Captan.	
Resumo: 	Protoplast isolation and regeneration were evaluated in two wild-type and two colour mutant strains of Metarhizium flavoviride. Cultivation in liquid medium, followed by mycelium treatment with Novozym 234 in the presence of KCl 0.7M as osmotic stabilizer, produced 5.05 x 10(6) to 1.15 x 10(7)x mL-1 protoplasts. The percentage of regeneration ranged from 6.65 to 27.92%. Following protoplast regeneration, one strain produced spontaneously stable morphological variant colonies. Although colonies with altered morphology have been reported in bacteria following protoplast regeneration, this is the first time that the same is described in a filamentous fungus. The original strain and one derived variant were tested for sensitivity to the fungicides benomyl and captan.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000100001	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132004000100001	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.47 n.1 2004	
Fonte: 	info:eu-repo/semantics/openAccess