Data/hora: 03/06/2024 01:20:04

Provedor de dados: 	Brazilian Archives of Biology and Technology	
País: 	Brazil	
Título: 	Viability of bull spermatozoa collected from the epididymis stored at 18-20°C	
Autores: 	Bertol,Melina Andrea Formighieri; Weiss,Romildo Romualdo; Thomaz-Soccol,Vanete; Kozicki,Luiz Ernandes; Fujita,Aline Silva; Abreu,Renata Azevedo de; Green,Kerriel Thandile.	
Data: 	2013-10-01	
Ano: 	2013	
Palavras-chave: 	Bull; Epididymis; Orchiectomy; Sperm; Room temperature; Viability.	
Resumo: 	The aim of this study was to evaluate the viability of bull spermatozoa collected from the cauda epididymis stored at 18-20°C, which were compared with semen collected by electro-ejaculation method and preserved at 5°C. Ten pairs of testes from Tabapuã bulls were removed by orchiectomy and stored for 6 (G6), 12 (G12), 18 (G18), 24 (G24) and 30 (G30) h at room temperature (18-20°C). Seven days before orchiectomy, semen was collected by electro-ejaculation method. The sperm parameters evaluated were: sperm motility, vigor, concentration, morphology and acrosome defects. Sperm motility declined (p&lt;0.05) when spermatozoa were stored for 30 h in the epididymis. The spermatozoa from the epididymis showed lower sperm motility than that of spermatozoa collected via electro-ejaculation. There was a little expressive decrease in sperm vigor and increased in morphological defects with storage time, but the acrosome integrity was not affected. Cold storage (5°C) maintained sperm viable for 15 to 40.8 h. Thus, it was possible to recover viable sperm with 41.25% of motility from the cauda epididymis stored at room temperature of 18-20°C for 30 h. There were differences between the ejaculated and epididymal sperm for the bulls and the conservation at 5°C allowed short-term preservation of the gametes.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500008	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132013000500008	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.56 n.5 2013	
Fonte: 	info:eu-repo/semantics/openAccess