Data/hora: 05/06/2024 13:06:23

Provedor de dados: | 52
País: | Brazil
Título: | Partial purification and characterization of xylanase produced by Penicillium expansum
Autores: | Querido,André Luiz de Souza; Coelho,Jorge Luiz Cavalcante; Araújo,Elza Fernandes de; Chaves-Alves,Virgínia Maria.
Data: | 2006-05-01
Ano: | 2006
Palavras-chave: | Partial purification; Xylan/xylanases; Penicillium expansum.
Resumo: | An extracellular xylanase was found to be the major protein in the filtrate culture of Penicillium expansum when grown on 0.3 % wheat bran, which showed no xylanase multiplicity. The enzime was partial purified by.ammonium sulfate fractioning, molecular exclusion chromatography, ultrafiltration and anion exchange chromatography. The protein eluation profile showed only one form of xylanase that was partially characterized. The activity of purified xylanase was optimal at pH 5.5 and 40 ºC. The enzyme was stable at pH between 5.5 and 6.5 and temperatures between 20-40 ºC. The enzyme showed a Km of 3.03 mM and Vmax of 0.027 mumol min-1 mug -1 of protein. The enzymatic activity was increased 31 % by Mg2+ and 28 % by Al3+.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400016
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132006000400016
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.49 n.3 2006
Fonte: | info:eu-repo/semantics/openAccess