Data/hora: 10/06/2024 12:18:33

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Radiation-induced Changes in the Electrophoretic Profile of Serum Albumin	
Autores: 	Lima,Celso Vieira; Campos,Tarcisio Passos Ribeiro.	
Data: 	2017-01-01	
Ano: 	2017	
Palavras-chave: 	Serum protein; Rats; Radiation; Albumin; Electrophoresis.	
Resumo: 	ABSTRACT Albumin protein profiles were investigated in electrophoresis system in relation to the whole body exposition to the radiation. Two groups of rats Wistar were set up as the control (CG) and the irradiated one (IG). The IG was exposed to Co-60 at a dose of 5 Gy. After a 72-hour exposition, 300 μL of blood was collected in the inferior vena cava, renal, jugular, hepatic, and pulmonary veins and the serum separated. The albumin protein was identified by vertical electrophoresis in acrylamide Commassi blue or silver stained. The calibration procedure was applied to albumin samples with well-known concentrations. The mathematical correlation was developed involving electrophoretic parameters of band intensities and sizes from gel representation, providing values of protein concentrations in comparison with standard bands with known concentrations. There were significant differences in the physiological concentrations in the jugular and pulmonary sites in relation to renal and cava regional sites. Significant differences induced by radiation in serum albumin concentration were also found in hepatic and jugular sites. Alteration of albumin concentration was found as a nearly effect from whole body irradiation. This phenomenon points out to alterations in cell metabolism in the liver justified by a possible indication of proteomics damage from radiation.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100444	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/1678-4324-2016160246	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.60 2017	
Fonte: 	info:eu-repo/semantics/openAccess