Data/hora: 05/06/2024 12:55:50

Provedor de dados: | 52
País: | Brazil
Título: | Effect of raffinose and ultrasound pulses on invertase release by free and immobilized Saccharomyces cerevisiae in loofa (Luffa cylindrica) sponge
Autores: | Marques,Leila Larisa Medeiros; Buzato,João Batista; Celligoi,Maria Antonia Pedrine Colabone.
Data: | 2006-11-01
Ano: | 2006
Palavras-chave: | Immobilization; Invertase; Luffa cylindrica; Raffinose; Saccharomyces cerevisiae; Ultrasound.
Resumo: | This study investigated the effect of raffinose and ultrasound pulses on invertase release from free S. cerevisiae and S. cerevisiae immobilized in Luffa cylindrica. The free cell culture was submitted to 2% raffinose pulse and irradiated for 2 minutes at 0.12 and 0.46 h-1 dilution rates. The immobilized cell culture was submitted to raffinose pulse and irradiated for 1, 2 and 4 minutes, at 0.10 h-1 dilution rate. In immobilized cells, the raffinose pulse increased the invertase activity from 5.38 to 7.27 U/mg. Ultrasound application in free cell culture at the 0.12 h-1 dilution rate gave the best results. The activity varied from 25.08 to 29.38 U/mg while the increase in immobilized cells was from 5.22 to 9.70 U/mg when sonicated for two minutes. These results showed that ultrasound application in continuous culture could have great potential for application in biotechnological techniques.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700003
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132006000700003
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.49 n.6 2006
Fonte: | info:eu-repo/semantics/openAccess