Data/hora: 11/06/2024 14:34:04

Provedor de dados: 	Brazilian Archives of Biology and Technology	
País: 	Brazil	
Título: 	Recovery of phytase produced by solid-state fermentation on citrus peel	
Autores: 	Rodríguez-Fernández,Daniel Ernesto; Rodríguez-León,José Angel; Carvalho,Júlio Cesar de; Thomaz-Soccol,Vanete; Parada,José Luis; Soccol,Carlos Ricardo.	
Data: 	2010-12-01	
Ano: 	2010	
Palavras-chave: 	Leaching; Recovery multistage; Phytase; Solid state fermentation; Kinetic models.	
Resumo: 	The extraction of phytase produced by solid-state fermentation of citrus peel was studied employing a multistage leaching process. It was observed that the extracts containing EDTA retained over 90% of phytase activity at room temperature after 24 h after the leaching. A fractional design 2² (with 4 replicates at the central point) was carried out for testing the pH and agitation as process independent factors. Only the interaction between the pH and agitation showed a significant influence. These factors were optimized with a central composite design. Agitation at 300 rpm and pH at 5.0 were the best conditions to extract the enzyme from solid matrix. The modeling of the process indicated that diffusivity of the enzyme in the solvent was the controlling mechanism. The corresponding kinetic constant and saturation concentration in this process were 0.89 min-1 and 4.0 IU/mL, respectively. The multistage process indicated that after two steps, it was possible to recover 85% of total enzyme produced.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600026	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132010000600026	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.53 n.6 2010	
Fonte: 	info:eu-repo/semantics/openAccess