Data/hora: 06/06/2024 02:35:35

Provedor de dados: | 52
País: | Brazil
Título: | Molecular Insights into the Genetic Diversity of Garcinia cambogia Germplasm Accessions
Autores: | Tharachand,C; Immanuel Selvaraj,C; Abraham,Z.
Data: | 2015-10-01
Ano: | 2015
Palavras-chave: | Garcinia cambogia; Genetic diversity; Germplasm; Jaccard's coefficient; Malabar tamarind.
Resumo: | ABSTRACTIn this work, the genetic relationship among twelveGarcinia cambogia (Gaertn.) Desr. accessions were evaluated using Random Amplified Polymorphic DNA markers. The samples were part of the germplasm collected and maintained at NBPGR Regional station, Thrissur, India. Out of thirty RAPD primers used for screening, seven primers produced a total of 128 polymorphic markers in twelve accessions. The Polymorphic Information Content (PIC) ranged from 0.28 (OPA18) to 0.37 (OPA9) and Marker Index (MI) ranged between 3.61 (OPA12) and 5.93 (OPA3) among the primers used. Jaccard's coefficient of genetic similarity ranged between 0.07 and 0.64. The dendrogram constructed based on the similarity matrix generated from the molecular and morphological data showed the genetic relationship among the sampled accessions. Mantel matrix test showed a positive correlation (r = 0.49) between the cluster analysis of RAPD data and morphological data. The clustering pattern in the molecular dendrogram and Principle Coordinate Analysis (PCoA) showed that the genotypes were diverse, which was in congruence with the similarity index values and morphological dendrogram. High frequency of similarity values in the range of 0.11 to 0.17 suggested the existence of high genetic diversity among the accessions. The high level of genetic diversity among the studied accessions ofG.cambogia was also supported by the large variation in the morphological characters observed in the flowers, leaves, fruits and seeds of these sampled accessions. This is the first report for the molecular based genetic diversity studies for these accessions.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500765
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132015050197
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.58 n.5 2015
Fonte: | info:eu-repo/semantics/openAccess