Data/hora: 09/06/2024 02:49:34

Provedor de dados: | Brazilian Archives of Biology and Technology
País: | Brazil
Título: | Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion
Autores: | Diniz,Leandro Eugênio Cardamoni; Ruas,Claudete de Fátima; Carvalho,Valdemar de Paula; Torres,Fabrício Medeiros; Ruas,Eduardo Augusto; Santos,Melissa de Oliveira; Sera,Tumoru; Ruas,Paulo Maurício.
Data: | 2005-07-01
Ano: | 2005
Palavras-chave: | Coffea arabica; Genetic variability; RAPD; Restriction endonucleases.
Resumo: | The genetic variability of 40 accessions of_C. arabica was evaluated using a combination of the random amplified polymorphic DNA (RAPD) technique and restriction digestion of genomic DNA. The genetic variability and the relatedness among all accessions were initially evaluated using 195 RAPD primers which revealed a very low level of genetic variation. To improve the efficiency in the detection of polymorphism, the genomic DNA of all accessions were submitted to digestion with restriction endonucleases prior to PCR amplification. A total of 24 primers combined with restriction digestion of DNA rendered 318 bands, of which 266 (83.65%) were polymorphic. The associations among genotypes were estimated using UPGMA-clustering analysis. The accessions were properly clustered according to pedigree and agronomic features. The ability to distinguish among coffee accessions was greater for RAPD plus restriction digestion than for RAPD alone, providing evidences that the combination of the techniques was very efficient for the estimation of genetic relationship among_C. arabica genotypes.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000500002
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132005000500002
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.48 n.4 2005
Fonte: | info:eu-repo/semantics/openAccess