Data/hora: 03/06/2024 07:33:12

Provedor de dados: | 52
País: | Brazil
Título: | Extracellular amylase production of a thermotolerant Fusarium sp: isolated from Eastern Nigerian soil
Autores: | Nwagu,Tochukwu Nwamaka; Okolo,Bartholomew Ndubuisi.
Data: | 2011-08-01
Ano: | 2011
Palavras-chave: | Hydrolysis; Starch; Fungal growth; Amylase synthesis.
Resumo: | In this work, an α- amylase producing Fusarium sp. was isolated from the soil at 50 0C. Growth and enzyme production occurred at 30, 45 and 55 ºC. Soybean meal at 1% concentration, supplemented with 0.2% NH4Cl and 2.5% corn starch elicited the highest amylase yield. Optimum pH for the enzyme was pH 6.5 which retained over 60% of its activity after 24 h incubation at the pH range of 4.5-7.0. The enzyme showed high activity from 40-70 0C with optimal activity at 50 ºC and 78% activity was retained after incubation at 70 ºC for 30min. Catalytic function of the crude amylase was stimulated by Mg (136%), Ca (118%) and Zn (118%) at 2mM concentrations. The enzyme hydrolyzed cassava, potato and yam starches effectively.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400002
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132011000400002
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.54 n.4 2011
Fonte: | info:eu-repo/semantics/openAccess