Data/hora: 09/06/2024 01:48:15

Provedor de dados: 	52	
País: 	Brazil	
Título: 	High quality DNA from human papillomavirus (HPV) for PCR/RFLPs	
Autores: 	Wanderlei-Silva,Denise; Nobre,Mariana; Gonzaga,Rosa Silva; Viana,Luciana Silva; Ramalho Neto,Eduardo.	
Data: 	2005-01-01	
Ano: 	2005	
Palavras-chave: 	Human papillomavirus; DNA purification; PCR; RFLP.	
Resumo: 	The analysis of DNA in clinical samples for a secure diagnostic has become indispensable nowadays. Techniques approaching isolation of high molecular weigth DNA of HPV could lead to efficient amplification and early clinical diagnosis of the virus DNA by PCR (polymerase chain reaction). We describe a fast, non-toxical, efficient and cheap method for DNA isolation of human papilloma virus (HPV) from cervical smears using guanidine (DNAzol solution). A 450 bp DNA band correponding to the late region (L1) of the virus genome was detected by PCR, showing that the DNAzol extraction soluction generated a good viral DNA yield. The electrophoretic pattern after digestion with restriction endonucleases (RFLPs/PCR) revealed the predominance of HPV-16 and HPV-33 in the samples from the State of Alagoas, Brazil.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100006	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132005000100006	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.48 n.1 2005	
Fonte: 	info:eu-repo/semantics/openAccess