Data/hora: 06/06/2024 05:37:31

Provedor de dados: | 52
País: | Brazil
Título: | Validation of an ovarian biopsy method for monitoring oocyte development in the fat snook, Centropomus parallelus Poey, 1860 in captivity
Autores: | Ferraz,Eduardo Medeiros; Alvarez-Lajonchère,Luis; Cerqueira,Vinicius Ronzani; Candido,Sidinei.
Data: | 2004-08-01
Ano: | 2004
Palavras-chave: | Ovarian Biopsy; Fat Snook; Centropomus parallelus.
Resumo: | The validation of an ovarian biopsy method for in vivo assessment of oocyte maturation in Centropomus parallelus was studied. Diameters of intra-ovarian oocytes siphoned with cannula were analyzed fresh and preserved with 1% formalin in 0.7% NaCl solution. Oocytes in different stages were present along the ovaries, up to the tertiary yolk globule stage, which had a unimodal diameter frequency distribution. The oocyte diameter means were not significantly different at four sites along the ovaries (P &gt; 0.05). Samples obtained with cannula were representative of the ovary central portion, in vivo and in vitro samples of the seven females examined were not significantly different (P &gt; 0.05). An estimate of the coefficient of variation corrected for bias (P &lt; 0.05) for 8 repeated in vivo samples was 1.9 ± 0.6. The results demonstrated that for the species, the biopsy method was satisfactory, providing representative samples of the ovaries.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400018
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132004000400018
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.47 n.4 2004
Fonte: | info:eu-repo/semantics/openAccess