Data/hora: 10/06/2024 08:52:18

Provedor de dados: | 52
País: | Brazil
Título: | Initial segmentation patterns of microspores and pollen viability in soybean cultured anthers: indication of chromosome doubling
Autores: | Cardoso,Milena Barcelos; Kaltchuk-Santos,Eliane; Mundstock,Elsa Cristina de; Bodanese-Zanettini,Maria Helena.
Data: | 2004-09-01
Ano: | 2004
Palavras-chave: | Glycine max; FDA; Binucleate pollen grains; Multinucleate pollen grains; Spontaneous diploidization.
Resumo: | Anthers obtained from flowers buds of soybean cultivar IAS-5 were cultured in two basal culture media (B5 and B5 long). Cytological examinations of the in vitro anthers were performed during the first 20 days of culture to assay the viability (by propionic-carmine and fluorescein diacetate tests) and the stage of development of pollen grains. The frequencies of viable pollen grains varied significantly between bud sizes on the propionic-carmine analysis. The basal culture media and bud size had no clear effect on the frequencies of binucleate symmetrical and multinucleate pollen grains. Chromosome counts of metaphasic microspores throughout the culture period showed microspores with higher ploidy level in addition to normal chromosome number (n=20).
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000500005
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132004000500005
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.47 n.5 2004
Fonte: | info:eu-repo/semantics/openAccess