Data/hora: 13/06/2024 01:28:51

Provedor de dados: | Brazilian Archives of Biology and Technology
País: | Brazil
Título: | Development of an enzyme immunoassay for poliovirus antigens
Autores: | Hashimoto,Newton; Benati,Fabrício José; Lauretti,Flávio; Linhares,Rosa Elisa Carvalho; Nozawa,Carlos Mitihiko.
Data: | 2007-01-01
Ano: | 2007
Palavras-chave: | Enzyme immunoassay; Poliovirus antigen.
Resumo: | An indirect solid-phase enzyme immunoassay (EIA) was developed for the detection of poliovirus antigen. Virus antigen was obtained in LLC-MK2 cell cultures and used to prepare antibodies in rabbit and guinea pig. Antibodies were evaluated by double immunodiffusion and neutralization test. Optimal concentrations of guinea pig and rabbit immunoglobulins were determined by checkerboard titration. Microtitre plates were coated with 15.0 µg/ml guinea pig anti-polio immunoglobulin and rabbit anti-polio immunoglobulin at the concentration of 7.94 µg/ml was used as detecting antibody. The standard curve with eight different antigen concentrations in eight replicates resulted in a coefficient of variation (CV) between 2.1% to 7.8%. The dose-response relationship was determined by simple linear regression with a coefficient of correlation (R²) equal to 96.4%. The assay detected a minimum of 2.3 µg/ml poliovirus antigen.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100005
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132007000100005
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.50 n.1 2007
Fonte: | info:eu-repo/semantics/openAccess