Data/hora: 08/06/2024 11:42:48

Provedor de dados: | 52
País: | Brazil
Título: | Cell density and solvent are critical parameters affecting formazan evaluation in MTT assay
Autores: | Gasque,Kellen Cristina da Silva; Al-Ahj,Luana Polioni; Oliveira,Rodrigo Cardoso; Magalhães,Ana Carolina.
Data: | 2014-06-01
Ano: | 2014
Palavras-chave: | Cell viability; MTT assay; Fibroblast NIH/3T3; Solvents.
Resumo: | The aim of this study was to establish the more accurate protocol for fibroblast cell viability using MTT assay. NIH/3T3 fibroblasts were seeded at the following cell densities: 3.125x10³; 1.156x10(4); 3.125x10(4); 1.156x10(5) and 3.125x10(5) cells/cm². Following 24h of seeding, MTT was added to the wells. After 4h of the MTT addition, different solvents were added to solubilize the formazan crystals: 1) HCl/SDS group- 20% SDS and 0.01 M HCl; 2) EtOH/ HAc group-50% ethanol and 1% acetic acid; 3) DMSO group- 99.5% dimethyl sulfoxide; and 4) PropOH group- 99.5% isopropanol. The absorbance values were measured using a spectrophotometer at 570 nm. The data were analyzed by 2-way ANOVA (p&lt;0.05) and showed that the absorbance average varied according to the number of cells and solvents: HCl/SDS (0 to 0.13), EtOH/HAc (0 to 0.22), DMSO (0.76 to 1.31) and PropOH (0.66 to 1.04). The DMSO and PropOH groups presented the most appropriate protocols for NIH/3T3 fibroblasts cell viability, especially at the density of 1.156x10(4) cells/cm².
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300011
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132014005000007
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.57 n.3 2014
Fonte: | info:eu-repo/semantics/openAccess