Data/hora: 01/06/2024 19:16:04

Provedor de dados: | Brazilian Archives of Biology and Technology
País: | Brazil
Título: | Aqueous Extract of Terminalia chebula Induces Apoptosis in Lung Cancer Cells Via a Mechanism Involving Mitochondria-mediated Pathways
Autores: | Wang,Meiling; Yang,Limin; Ji,Musi; Zhao,Pengwei; Sun,Peng; Bai,Ruixia; Tian,Yunpeng; Su,Liping; Li,Cunbao.
Data: | 2015-04-01
Ano: | 2015
Palavras-chave: | T. chebula; Aqueous extract; Infrared spectroscopy; Apoptosis.
Resumo: | The current study was designed to evaluate the aqueous extract of Terminalia chebula activity, and the main pathway was detected on lung cancer by extracts of T. chebula. Aqueous extract of T. chebula was separated using a zeolite, and five fractions of T. chebula extract were obtained and analyzed by ultraviolet (UV) and infrared (IR) spectroscopy. Antiproliferative activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) methods against human lung cancer (A549) and mouse lung cancer cell line LLC. T. chebula acts by regulating the Bcl-2 family protein-mediated mitochondrial pathway detected by western blot. Fraction 4 of the T. chebula extract showed much function and was thus studied further. Fraction 4 increased the activation of caspase-3, induced PARP cleavage, and promoted cytochrome c release into the cytoplasm. These data suggest that T. chebula acts by regulating the Bcl-2 family protein-mediated mitochondrial pathway and provide evidence that T. chebula deserves further investigation as a natural agent for treating and preventing cancer.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200208
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-8913201400202
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.58 n.2 2015
Fonte: | info:eu-repo/semantics/openAccess