Data/hora: 09/06/2024 10:40:15

Provedor de dados: | 52
País: | Brazil
Título: | Direct RAPD evaluation of bacteria without conventional DNA extraction
Autores: | Araújo,Welington Luiz; Angellis,Derlene Attili de; Azevedo,João Lúcio.
Data: | 2004-07-01
Ano: | 2004
Palavras-chave: | Bacteria; Boiling method; DNA extraction; RAPD.
Resumo: | The present work reports successful DNA amplification of Pantoea agglomerans and Bacillus pumilus through Random Amplified Polymorphic DNA (RAPD). For this, template DNA was obtained without conventional DNA extraction. The procedure was as follows: cultures grown for 20 hours in 5 mL LB medium were centrifuged and the resulting preparation was suspended in TE buffer. After boiling, the cell suspension was diluted and 2.0 µl were used in reactions of 15 µl. The results showed no significant differences among the RAPD profile of the PCR reactions derived from the boiling and phenol extraction methods, suggesting the utilization of this method for genetic population analysis.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300006
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132004000300006
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.47 n.3 2004
Fonte: | info:eu-repo/semantics/openAccess