Data/hora: 06/06/2024 01:13:38

Provedor de dados: | Brazilian Archives of Biology and Technology
País: | Brazil
Título: | Partition of glucose oxidase from Aspergillus niger in aqueous two-phase systems based on salt and polyethylene glycol
Autores: | Singh,Jagdish; Verma,Neelam.
Data: | 2010-10-01
Ano: | 2010
Palavras-chave: | Aspergillus niger; Glucose oxidase; Aqueous two phase.
Resumo: | The aim of this work was to study the isolation of glucose oxidase (GOx) from Aspergillus niger in aqueous two phase system consisting of PEG 7500 (150g l-1), potassium phosphate (175 g l-1, K2HPO4 +KH2PO4) and glucose (10 gl-1), the enzyme was partitioned in polymer phase. By sequential extraction GOx (69.2%) was recovered in polymer phase by 11.8 fold purification, giving a yield of 129U mg protein-¹.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500007
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132010000500007
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.53 n.5 2010
Fonte: | info:eu-repo/semantics/openAccess