Data/hora: 10/06/2024 10:02:09

Provedor de dados: 	Brazilian Archives of Biology and Technology	
País: 	Brazil	
Título: 	Polyphenols From Cutch Tree (Acacia catechu Willd.): Normalize In Vitro Oxidative Stress and Exerts Antiproliferative Activity	
Autores: 	Kumar,Rakesh; Arora,Rohit; Mahajan,Jyoti; Mahey,Sonia; Arora,Saroj.	
Data: 	2017-01-01	
Ano: 	2017	
Palavras-chave: 	Acacia catechu; Antioxidant; Antiproliferative; UHPLC; MTT.	
Resumo: 	ABSTRACT Oxidative stress, being the main cause of most of the human diseases, has always been the highlight of research worldwide. This stress can be overcome by administration of natural polyphenols. The Acacia catechu Willd. has many refrences available in Ayurveda as important disease curative plant. Its leaves are investigated for ameliorating oxidative stress in present work. Leaves of A. catechu were extracted with 80% methanol to get methanol extract (AME). It was assessed for antioxidant activity using DPPH, ABTS, CUPRAC, ferric ion reducing, superoxide scavenging and peroxyl radical scavenging assays. DNA protective activity was also investigated using plasmid nicking assay. Further, antiproliferative activity was determined using MTT assay in various human cancer cell lines. The quantification of polyphenols was done by UHPLC analysis. Results confirmed that polyphenols of A. catechu were successful in normalizing oxidative stress. AME was found to be most effective in scavenging ABTS radicals while least effective in scavenging ferric ions. UHPLC analysis showed abundance of ellagic acid, rutin and quercetin in AME. Further, AME showed maximum antiproliferative activity against Hep G2 cancer cells. It is concluded that the polyphenols from A. catechu effectively remediates oxidative stress and hence can be used in curing numerous dreadful diseases.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100316	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/1678-4324-2017160728	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.60 2017	
Fonte: 	info:eu-repo/semantics/openAccess