Data/hora: 10/06/2024 11:58:35

Provedor de dados: | Brazilian Archives of Biology and Technology
País: | Brazil
Título: | A rapid and reliable method for the clonal isolation of Acanthamoeba from environmental samples
Autores: | Zanella,Janice; Costa,Sergio Olavo Pinto da; Zacaria,Jucimar; Echeverrigaray,Sergio.
Data: | 2012-02-01
Ano: | 2012
Palavras-chave: | Acanthamoeba; Monocystic culture; Micromanipulation.
Resumo: | Acanthamoeba are abundant in a wide range of environments, and some species are responsible for cutaneous infections, keratitis, and granulomatous amoebic encephalitis (GAE). The conventional detection and isolation of amoeba from clinical and environmental samples involves sampling and culture on non-nutrient Ágar medium. Although efficient, this system requires several transfers in order to eliminate contaminants, and is not appropriate for the isolation of individual amoeba from samples with a biodiverse community. In this study we propose an alternative method for the isolation of monocystic clones of Acanthamoeba. The propose method involves sampling, enrichment, encystment induction, and direct cysts micromanipulation and culture on Ágar plates.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100001
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132012000100001
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.55 n.1 2012
Fonte: | info:eu-repo/semantics/openAccess