Data/hora: 11/06/2024 06:40:48

Provedor de dados: | Brazilian Archives of Biology and Technology
País: | Brazil
Título: | Development and Evaluation of an Indirect ELISA: Serological Survey to Detect Specific Antibodies to Bovine Herpesvirus 4
Autores: | Krüger,Ernesto Renato; Penha,Tânia Regina; Hummelgen,Fernanda Cristina; Agottani,Jorge Bacila; Reva,Demétrio; Gonçalves,Robson; Thomaz-Soccol,Vanete.
Data: | 2015-10-01
Ano: | 2015
Palavras-chave: | BoHV-4; Serology; Diagnostic; Cattle; Epidemiology.
Resumo: | ABSTRACTThe aim of this work was to develop and evaluate an indirect enzyme-linked immunosorbent assay (ELISA) and made a serological screening for specific antibodies to BoHV-4. Bovine serum samples were collected from different Brazilian states and evaluated for the presence of antibodies for BoHV-4 and BoHV-1. The serological results obtained showed that the indirect ELISA assay could be applied for the detection of specific antibodies for BoHV-4. The ELISA test allowed concluding that BoHV-4 is present in bovines in every Brazilian state from which serum samples were collected. The ELISA assay here standardized proved to be useful for the epidemiological studies and showed a positivity range from 1.8 to 66% in Brazil.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500725
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132015050187
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.58 n.5 2015
Fonte: | info:eu-repo/semantics/openAccess