Data/hora: 06/06/2024 03:35:20

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Biochemical characterisation of a glucoamylase from Aspergillus niger produced by solid-state fermentation	
Autores: 	Slivinski,Christiane Trevisan; Machado,Alex Vinicius Lopes; Iulek,Jorge; Ayub,Ricardo Antônio; Almeida,Mareci Mendes de.	
Data: 	2011-06-01	
Ano: 	2011	
Palavras-chave: 	Glucoamylase; Aspergillus niger; Solid-state fermentation; Biochemical characterization; Purification.	
Resumo: 	In this work, glucoamylase was produced by Aspergillus niger in solid-state fermentation. The enzyme was partially purified by ammonium sulphate precipitation and ion exchange and gel filtration chromatographies. Its molecular mass was estimated as 118.17 kDa by electrophoresis. The partially purified enzyme had an optimum pH range of 4.5-5.0 and an optimum temperature of 60 °C, with average activity 152.85 U mL-1. Thermal and pH stability assays with the crude extract showed that more than 60 % of the activity remained at pH 4.6 and 60 °C, even after an exposition to these conditions longer than 24 h. Yet, after purification, the enzyme was stable at these for at least 4 h, which indicated that its purification for use in starch saccharification was inadvisable. K M and Vmax were 0.34 mg mL-1 and 160.22 U mL-1, respectively.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300018	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132011000300018	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.54 n.3 2011	
Fonte: 	info:eu-repo/semantics/openAccess