Data/hora: 08/06/2024 03:34:58

Provedor de dados: 	Brazilian Archives of Biology and Technology	
País: 	Brazil	
Título: 	LC50 of the Peptide Produced by the Entomopathogenic Fungus Nomuraea rileyi (Farlow) Samson Active Against Third Instar Larvae of Anticarsia gemmatalis (Lep.: Noctuidae)	
Autores: 	Onofre,Sideney Becker; Riveros Gonzalez,Raul; Messias,Cláudio Luiz; Azevedo,João Lúcio; Barros,Neiva Monteiro de.	
Data: 	2002-09-01	
Ano: 	2002	
Palavras-chave: 	Anticarsia gemmatalis; Nomuraea rileyi; Integrated control; Secondary metabolite; Toxins.	
Resumo: 	The entomopathogenic fungus Nomuraea rileyi (Farlow) Samson produced a peptide active against Anticarsia gemmatalis 3rd instar larvae. To produce this peptide, N. rileyi was cultivated aerobically in Saboraud, maltose, yeast-extract broth at 26 ± 1ºC for 12 days, after which the medium was filtered and separated in a liquid/liquid extractor, concentrated and the peptide purified chromatographically. The crystals obtained were kept refrigerated until needed for LC50 analysis. The LC50 of this peptide against A. gemmatalis 3rd instar larvae was determined in triplicate experiments using solutions containing 1.0, 0.2, 0.1, 0.01, 0.001 and 0.0001 mg/ml of N. rileyi peptide. The results of these experiments were used to calculate a linear equation in which Y = 6,81176 + 1,01382 * LOGx , giving a LC50 value of 0.0163 mg/ml.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000300004	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132002000300004	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.45 n.3 2002	
Fonte: 	info:eu-repo/semantics/openAccess