Data/hora: 03/06/2024 16:35:36

Provedor de dados: | 52
País: | Brazil
Título: | Semliki forest virus as a vector: pros and cons for its use in biopharmaceuticals production
Autores: | Núñez,Eutimio Gustavo Fernández; Jorge,Soraia Attie Calil; Astray,Renato Mancini; Rezende,Alexandre Gonçalves de; Costa,Bruno Labate Vale da; Monteiro,Daniella Cristina Ventini; Pereira,Carlos Augusto; Tonso,Aldo.
Data: | 2013-10-01
Ano: | 2013
Palavras-chave: | BHK-21; Large-scale bioprocesses; Mammalian cells; Recombinant proteins; SFV; Transient expression.
Resumo: | The number of biopharmaceuticals for medical and veterinarian use produced in mammalian cells is increasing year after year. All of them are obtained by stable recombinant cell lines. However, it is recognized that transient gene expression produces high level expression in a short time. In that sense, viral vectors have been extensively used for producing recombinant proteins on lab-scale. Among them, Semliki Forest virus is commonly employed for this purpose. This review discusses the main aspects related to the use of Semliki Forest virus technology as well as its advantages and drawbacks which limit currently its utilization in biopharmaceutical industry on large-scale.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500018
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132013000500018
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.56 n.5 2013
Fonte: | info:eu-repo/semantics/openAccess