Data/hora: 10/06/2024 09:20:20

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Investigation of the possible role of Chlamydophila abortus in reproductive failures in nrazilian herds of domestic ruminants	
Autores: 	Silva-Zacarias,Francielle Gibson da; Alfieri,Amauri Alcindo; Spohr,Kledir Anderson Hofstaetter; Lima,Bruna Azevedo de Carvalho; Oliveira,Rosângela Claret de; Turilli,Carlo; Lunardi,Michele; Otonel,Rodrigo Alejandro Arellano; Freitas,Julio Cesar de.	
Data: 	2009-11-01	
Ano: 	2009	
Palavras-chave: 	Cattle; Small ruminants; Chlamydophila abortus; Abortion; Vaginal mucus; Semen.	
Resumo: 	Chlamydophila abortus (C. abortus) infection is related to reproductive failure in domestic ruminants. Although it has not been well characterized worldwide, this pathogen has already been identified in some European countries and in the USA. In Brazil, preliminary studies have shown serological evidence of C. abortus infection in herds with low antibody prevalence. Until now, the identification of C. abortus in biological samples from females presenting reproductive failures has not been described in Brazilian herds of domestic ruminants. The aim of this study was to evaluate the presence of the C. abortus in a collection of abortions from cattle (n=85), sheep (n=12), and goats (n=8), in samples of vaginal mucus from cows (n=13), sheep (n=90), and goats (n­=20), and in semen from sheep (n=10) and goats (n=5). The specimens (n=243) were evaluated using a PCR assay developed to amplify the 16S-23S rRNA intergenic space of C. abortus. A PCR assay with an internal control, which amplifies a fragment from the ND5 gene of bovine mitochondrial DNA, was used in order to evaluate the efficiency of the DNA extraction and of the PCR reaction. All biological samples (n=243) included in this study were negative for C. abortus in the PCR assay. The internal control enabled the amplification of a product from the bovine mitochondrial ND5 gene in all cattle abortion samples (n=85). Given the serological evidence indicating the presence of C. abortus infection in Brazilian herds of domestic ruminants, and considering the wide sampling evaluated, the failure to identify C. abortus in this survey suggests that the frequency of clinical signs in infected animals may be low or even absent.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000700015	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132009000700015	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.52 n.spe 2009	
Fonte: 	info:eu-repo/semantics/openAccess