Data/hora: 08/06/2024 07:11:57

Provedor de dados: | 52
País: | Brazil
Título: | Real time PCR and importance of housekeepings genes for normalization and quantification of mRNA expression in different tissues
Autores: | Rebouças,Emanuela de Lima; Costa,José Jackson do Nascimento; Passos,Maria Juliane; Passos,José Renato de Sousa; Hurk,Robert van den; Silva,José Roberto Viana.
Data: | 2013-02-01
Ano: | 2013
Palavras-chave: | PCR; Normalization; Reference genes; Gene expression; MRNA.
Resumo: | The aim of this review was to evaluate the importance of the real-time PCR (qRT-PCR) as a technique for mRNA expression analysis in different tissues. Real-time PCR is widely used for quantification of mRNA levels and is a fundamental tool for basic research, molecular medicine and biotechnology.Genes of references are expressed in a wide variety of tissues and cells with minimal variations in their expression levels, and thus are used to normalize data of mRNA quantification. Software programs, such as geNorm, BestKeeper and NormFinder, have been developed to perform the normalization of data, which help to choose the most stable reference gene. Several genes, such as GAPDH, β-actin, β-tubulin, PGK, UBQ, RPL-19 and 18S rRNA have been suggested as standards in PCR studies, but these genes can have variation in their expression in different tissues, reinforcing the idea that there is no ideal reference gene.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000100019
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132013000100019
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.56 n.1 2013
Fonte: | info:eu-repo/semantics/openAccess