Data/hora: 09/06/2024 11:37:37

Provedor de dados: 	Brazilian Archives of Biology and Technology	
País: 	Brazil	
Título: 	Increasing Scopolamine content in Hairy Roots of Atropa belladonna using Bioreactor	
Autores: 	Habibi,Peyman; Piri,Khosro; Deljo,Ali; Moghadam,Yaser Ahmadi; Ghiasvand,Taiebeh.	
Data: 	2015-04-01	
Ano: 	2015	
Palavras-chave: 	Atropa belladonna; Hairy root; Scopolamine; Bioreactor.	
Resumo: 	The aim of this study was to use the, hairy root system for increasing the scopolamine content in Atropa belladonna. Agrobacterium rhizogenes ATCC15834 was utilized to produce hairy roots. The culture was carried out in a 1.5-l bioreactor using the inoculum size of 0.5 g fr. wt of 10-day-old hairy roots and various parameters, including agitation, aeration, conductivity and the consumption of sucrose were evaluated. Results revealed that the highest amount of scopolamine production (1.59 mg/g-1 dry wt) occurred in the bioreactor with aeration and agitation 1.25 vvm (volume per volume per minute) and 70 rpm, respectively. Study of conductivity and the consumption of sucrose showed that the highest amount of sucrose consumption and the highest amount of minerals consumption also was at 1.25 vvm and 70 rpm. Transgenic hairy root lines were confirmed by polymerase chain reaction (PCR).	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200166	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-8913201400276	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.58 n.2 2015	
Fonte: 	info:eu-repo/semantics/openAccess