Data/hora: 13/06/2024 18:55:36

Provedor de dados: | 52
País: | Brazil
Título: | Polyamine patterns in haploid and diploid tobacco tissues and in vitro cultures
Autores: | Carone,Sílvia Bicudo; Santa-Catarina,Claudete; Silveira,Vanildo; Floh,Eny Iochevet Segal.
Data: | 2010-04-01
Ano: | 2010
Palavras-chave: | Biotechnology; Nicotiana tabacum; Ploidy; Putrescine; Spermidine; Spermine.
Resumo: | The aim of this work was to determine PAs levels in pith tissues and callus cultures from haploid and diploid tobacco plants, explanted from the apical and basal regions of the stem. These explants were cultured in an RM-64 medium supplied with IAA and kinetin, under light or in the dark, during successive subcultures. PAs levels followed a basipetal decrease in diploid and an increase in haploid, pith tissues. A similar pattern of total PAs (free + conjugated) was observed for the callus of diploid and haploid plants maintained in the light, and for the haploid callus in the dark, whereas the diploid callus in the dark showed a constant increase in total PAs levels until the end of culture. The PA increase in the diploid callus in the dark was related to free Put levels increase. The ploidy status of the plants could express different PA gradients together with the plant pith and in vitro callus cultures.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200020
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132010000200020
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.53 n.2 2010
Fonte: | info:eu-repo/semantics/openAccess