Data/hora: 03/06/2024 16:59:08

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Validation of computationally predicted substrates for laccase	
Autores: 	Reena,; Dhall,Purnima; Kumar,Rita; Kumar,Anil.	
Data: 	2014-10-01	
Ano: 	2014	
Palavras-chave: 	Biodegradation; In silico screening; Laccase; Xenobiotics.	
Resumo: 	Present study reports the validation (oxidation) of computationally predicted oxidation of xenobiotic contaminants by commercially available pure laccase from Trametes versicolor. Selected contaminants were predicted as potential targets for laccase oxidation by using in-silico docking tool. The oxidation by laccase was measured by change in absorbance at specific λ max of each compound. Sinapic acid and tyrosine were taken as positive and negative controls, respectively. Oxidation was observed in m-chlorophenol, 2,4 di-chlorophenol, 2,4,6 tri-chlorophenol, captan, atrazine and thiodicarb, except malathion, which showed no activity. It could be speculated that the predicted substrates showing oxidation shared homology at structural and chemical level with positive control compounds. In case of malathion, structural non-homology with sinapic acid could be attributed to its inactivity towards laccase that required further structural analysis. Thus, a remediation tool proposing an advanced remediation approach combining the application of theoretical in-silico method and subsequent experimental validation using pure laccase could be proposed. As number and type of xenobiotics increase, the unfeasibility to screen them experimentally for bioremediation also rise. This approach would be useful to reduce the time and cost required in other screening methods.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500803	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-8913201402239	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.57 n.5 2014	
Fonte: 	info:eu-repo/semantics/openAccess