Data/hora: 16/06/2024 07:49:03

Provedor de dados: 	52	
País: 	Brazil	
Título: 	Purification of papain from fresh latex of Carica papaya	
Autores: 	Monti,Rubens; Basilio,Carmelita A.; Trevisan,Henrique C.; Contiero,Jonas.	
Data: 	2000-01-01	
Ano: 	2000	
Palavras-chave: 	Papain; Carica papaya; Cysteine proteinase; Proteolytic enzymes; Purification of papain.	
Resumo: 	In the present study we wish to report a method of crystallizing papain from fresh papaya latex which gave higher yields than previously reported. This method does not involve the use of sulphydryl reagents. The papain thus obtained is practically pure and shows a single band when submitted to electrophoresis on polyacrylamide gel, and is identical to the papain obtained by other methods. In routine enzymatic assays, specific activity was measured using Z-gly-pNP and BAEE as substrates. Papain crystallized by this method, without the use of high concentrations of salts or thiol-containing substances such as cysteine and dithiothreitol, is obtained in the form of a complex with natural inhibitors existent in latex which can be removed by dialysis.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500009	
Editor: 	Instituto de Tecnologia do Paraná - Tecpar	
Relação: 	10.1590/S1516-89132000000500009	
Formato: 	text/html	
Fonte: 	Brazilian Archives of Biology and Technology  v.43 n.5 2000	
Fonte: 	info:eu-repo/semantics/openAccess