Data/hora: 16/06/2024 09:22:31

Provedor de dados: | 52
País: | Brazil
Título: | Hydrolysis of whey lactose by immobilized β-Galactosidase
Autores: | Mariotti,Marcela Panaro; Yamanaka,Hideko; Araujo,Angela Regina; Trevisan,Henrique Celso.
Data: | 2008-12-01
Ano: | 2008
Palavras-chave: | Β-galactosidase; Immobilization; Lactase; Whey; Lactose; Hydrolysis.
Resumo: | Hydrolysis of whey lactose to glucose and galactose by immobilized galactosidase comes as an alternative to enlarge the possibilities of commercial use of this feedstock. To be applied at industrial scale, the process should be performed continuously .This work aimed to study the hydrolysis of whey lactose by an immobilized enzyme reactor. b-Galactosidase from Aspergillus oryzae was immobilized on silica and activity and stability were evaluated. The best immobilization results were attained by using glutaraldehyde as support's activator and enzyme stabilizer. The optimized enzyme proportion for immobilization was 15-20 mg g-1 of support. Treatments of whey were performed (microfiltration, thermal treatment and ultrafiltration), seeking the elimination of sludge, and the effects on operating the fixed bed reactor were evaluated. Ultrafiltration was the best treatment towards a proper substrate solution for feeding the reactor.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600019
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132008000600019
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.51 n.6 2008
Fonte: | info:eu-repo/semantics/openAccess