Data/hora: 09/06/2024 14:38:07

Provedor de dados: | Brazilian Archives of Biology and Technology
País: | Brazil
Título: | Evaluation of gelling agents on anther culture: response of two soybean cultivars
Autores: | Cardoso,Milena Barcelos; Bodanese-Zanettini,Maria Helena; Mundstock,Elsa Cristina de; Kaltchuk-Santos,Eliane.
Data: | 2007-11-01
Ano: | 2007
Palavras-chave: | Anther culture; Glycine max; Culture media; Binucleate pollen grains; Multinucleate/multicellular structures; Callus formation.
Resumo: | Anthers of two soybean cultivars were cultured in B5 long basal culture media gelled with agarose or PhytagelTM. Cytological examinations of the anthers were carried out during the first 45 days of culture to assay the viability and developmental stage of microspores. Frequency of callus formation was recorded at 45 days of culture. The analysis of variance of the microspore viability assay showed significant Cultivar X Gelling Agent X Day of Culture interactions. The frequencies of viable microspores decreased significantly with time of culture, within each cultivar and gelling agent tested. The interaction Day X Cultivar was significant for the frequencies of binucleate symmetrical grains and multinucleate/multicellular structures. The effect of gelling agents on the frequency of binucleate symmetrical pollens grains and multinucleate/multicellular structures was not significant. About the frequencies of calli and embryogenic calli formed, a significant difference was detected between the cultivars (IAS5= 14.8% and BRS 133=6.6%). Gelling agents showed no effect over these frequencies.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700004
Editor: | Instituto de Tecnologia do Paraná - Tecpar
Relação: | 10.1590/S1516-89132007000700004
Formato: | text/html
Fonte: | Brazilian Archives of Biology and Technology  v.50 n.6 2007
Fonte: | info:eu-repo/semantics/openAccess