Data/hora: 11/06/2024 13:23:51

Provedor de dados: | 53
País: | Brazil
Título: | Assessment of EST-SSR markers for genetic analisys on coffee
Autores: | Missio,Robson Fernando; Caixeta,Eveline Teixeira; Zambolim,Eunize Maciel; Pena,Guilherme Ferreira; Ribeiro,Ana Paula; Zambolim,Laércio; Pereira,Antônio Alves; Sakiyama,Ney Sussumu.
Data: | 2009-09-01
Ano: | 2009
Palavras-chave: | DNA markers; Coffea sp.; UPGMA; Amova; Microsatellite markers.
Resumo: | EST-SSR markers were used to investigate the genetic diversity among and within coffee populations, to explore the possibility of their use for fingerprinting of cultivars and to assist breeding programs. Seventeen markers, developed from ESTs (Expressed Sequence Tags) from the Brazilian Coffee Genome Project, were used. All markers showed polymorphism among the genotypes assessed. The average number of allele per primer was 5.1. The highest polymorphisms were found within C. canephora (88.2%) and rust-resistant varieties (35.3%). About 29.4% of the markers differentiated C. arabica from Híbrido de Timor; it was also possible to identify those closest and farthest from C. arabica . The analysis of population-grouped genotypes revealed a 64.0% genetic diversity among and a 36.0% genetic diversity within populations. The differentiation index was 0.637. Six markers distinguished four rust-resistance varieties, showing their fingerprinting potential. These results demonstrate the usefulness of EST-SSR markers for cross orientation, in diversity and introgression studies, and in genetic mapping.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052009000300003
Editor: | Instituto Agronômico de Campinas
Relação: | 10.1590/S0006-87052009000300003
Formato: | text/html
Fonte: | Bragantia  v.68 n.3 2009
Fonte: | info:eu-repo/semantics/openAccess