Data/hora: 02/06/2024 10:51:14

Provedor de dados: | Bragantia
País: | Brazil
Título: | Rice genotypes for drought tolerance: morphological and transcriptional evaluation of auxin-related genes
Autores: | Madabula,Frederico Pedro; Santos,Railson Schreinert dos; Machado,Nata; Pegoraro,Camila; Kruger,Mariana Madruga; Maia,Luciano Carlos da; Sousa,Rogerio Oliveira de; Oliveira,Antonio Costa de.
Data: | 2016-12-01
Ano: | 2016
Palavras-chave: | Oryza sativ; RT-qPCR; Root; Abiotic stress.
Resumo: | ABSTRACT Drought stress affects crop quality and productivity. The challenge of increasing food availability for a growing worldwide demand relies on the development of tolerant cultivars that will need to be adapted to arid and semi-arid areas. In order to help the understanding of rice response to stress, the phenotypic response of 6 Brazilian rice cultivars and 2 different crosses between them were characterized under drought conditions. Since gene regulation is an important part of root morphological responses to stressful conditions, 4 genes related to auxin response and root modifications (OsGNOM1 CRL4, OsIAA1, OsCAND1 and OsRAA1) were evaluated. The expression of these genes was analyzed in stressed rice using public available microarray data and then through real-time quantitative polymerase chain reaction (RT-qPCR), in the 6 phenotypically evaluated Brazilian genotypes under standard conditions (absence of stress). Our results show that all genotypes lengthened its roots in response to drought, specially the 2 hybrids. The expression of these genes is modified in response to stress, and OsRAA1 has a very special behavior, constituting a target for future studies. Further steps include the study of polymorphisms in these genotypes in order to understand if differences in these genes or in regulatory regions can be associated with differences in root system architecture and/or stress tolerance.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052016000400428
Editor: | Instituto Agronômico de Campinas
Relação: | 10.1590/1678-4499.562
Formato: | text/html
Fonte: | Bragantia  v.75 n.4 2016
Fonte: | info:eu-repo/semantics/openAccess