Data/hora: 17/06/2024 15:43:26

Provedor de dados: 	54	
País: 	Brazil	
Título: 	TP53 mutations and loss of heterozygosity of chromosome 17 in colorectal tumors	
Autores: 	Yamamoto,Lidia; Lopes,Ademar A.; Harb-Gama,Angelito; Nagai,Maria A.	
Data: 	1996-01-01	
Ano: 	1996	
Palavras-chave: 	TP53 mutations; Heterozygosity; Chromosome 17; Colorectal tumors.	
Resumo: 	The incidence of TP53 point mutations and loss of heterozygosity (LOH) of chromosome 17 in colorectal tumors was determined in a group of Brazilian patients. We screened DNA samples from tumors and distal normal mucosa of 39 patients with colorectal cancer, for TP53 mutations by PCR-SSCP (single-strand conformation polymorphism) analysis. Chromosome 17 LOH was investigated using six PCR-based polymorphic markers and one VNTR probe. TP53 mutations were demonstrated in 15/39 of the cases. Mutations were distributed among all exons examined (five to eight), the majority of them being G/C to A/T transitions. LOH of chromosome 17p and 17q was detected in 70 and 46% of the tumors, respectively. There was a significant association between TP53 mutations and LOH in chromosome 17p (P = 0.0035) and 17q (P = 0.03). Although no correlation was observed between TP53 genetic alterations and clinical/ pathological characteristics, the association of TP53 mutations with loss of both chromosome 17 arms may indicate that TP53 inactivation provokes an unstable phenotype in tumor cells in colorectal tumors.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551996000400022	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S0100-84551996000400022	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Genetics  v.19 n.4 1996	
Fonte: 	info:eu-repo/semantics/openAccess