Data/hora: 04/06/2024 11:16:57

Provedor de dados: 	Brazilian Journal of Infectious Diseases	
País: 	Brazil	
Título: 	Comparison of multiplex PCR with serogrouping and PCR-RFLP of fliC gene for the detection of enteropathogenic Escherichia coli (EPEC)	
Autores: 	Bouzari,Saeid; Aslani,Mohammad M; Oloomi,Mana; Jafari,Anis; Dashti,Amir.	
Data: 	2011-08-01	
Ano: 	2011	
Palavras-chave: 	Diarrhea; Enteropathogenic Escherichia coli; Polymerase chain reaction.	
Resumo: 	Enteropathogenic Escherichia coli (EPEC) comprise one of the six categories of diarrhoeagenic E. coli (DEC). EPEC is subgrouped into typical (tEPEC) and atypical (aEPEC). The identification of DEC cannot be based only on cultural and biochemical criteria, since they are indistinguishable from the non-pathogenic E. coli commonly found in human feces. Several PCR methods, with both single and multiple target genes, have been reported for detecting the different DEC pathotypes. In the present study five hundred E. coli isolates from children with diarrhea were subjected into multiplex PCR. Furthermore the strains were typed serologically with O antisera and their fliC gene was characterized by PCR-RFLP. The results obtained revealed that overall 41 (8.2%) isolates could be detected as EPEC by this multiplex PCR assay. Of these isolates; 27 (66%) were typical (escv+, bfp+) and 14 (34%) atypical EPEC (escv+, bfp-). None of these 41 isolates contained the Stx1 and Stx2 genes. Among 37 (90%) typeable strains, nine different serogroups were present. The most common serogroups were O111, followed by O86, O55 and O119 and 10 different H types were found among these isolates. The multiplex PCR assay was found to be rapid and reliable in comparison to serological test; especially when screening the large number of isolates.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400011	
Editor: 	Brazilian Society of Infectious Diseases	
Relação: 	10.1590/S1413-86702011000400011	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Infectious Diseases  v.15 n.4 2011	
Fonte: 	info:eu-repo/semantics/openAccess