Data/hora: 11/06/2024 04:14:58

Provedor de dados: | 55
País: | Brazil
Título: | Comparison of a rapid cytomegalovirus pp65 antigenemia assay revealed by immunofluorescence to an in-house assay revealed by immunoperoxidase for diagnosis in solid organ transplant recipient patients
Autores: | Carraro,Emerson; Pasternak,Jacyr; Perosa,Ana; Siqueira,Itacy; Martino,Marines Dalla Vale.
Data: | 2010-06-01
Ano: | 2010
Palavras-chave: | Cytomegalovirus; Antigenemia assay; Immmunoperoxidase; Immunofluorescence.
Resumo: | Cytomegalovirus (CMV) antigenemia is still one of the two major assays available for diagnosis and monitoring of CMV infections. A commercial rapid test recently available in Brazil for quantification of human cytomegalovirus pp65 antigenemia revealed by immunofluorescence technique was compared with the original in-house method revealed by immunoperoxidase in patients receiving solid organ transplants. Of 80 blood samples tested for CMV antigenemia, 34 (42.5%) were positive: commercial assay detected 33 (97%) and in-house assay detected 20 (58.8%) samples. The numbers of positive cells in the two assays were different, with a median of 4.5 and 12 positive cells obtained by in-house and commercial kit, respectively. Discrepancies between assays occurred in 15 specimens from patients with low-grade antigenemia (median 6 positive cells). The assay-time was reduced in approximately 50% compared to in-house methodology. In conclusion, besides comparable results obtained for both assays, the commercial antigenemia assay provides more rapid and sensitive results.
Tipo: | Info:eu-repo/semantics/other
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000300023
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1590/S1413-86702010000300023
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.14 n.3 2010
Fonte: | info:eu-repo/semantics/openAccess