Data/hora: 03/06/2024 02:59:19

Provedor de dados: | Brazilian Journal of Infectious Diseases
País: | Brazil
Título: | Laboratory tests in the detection of extended spectrum beta-lactamase production: National Committee for Clinical Laboratory Standards (NCCLS) screening test, the E-test, the double disk confirmatory test, and cefoxitin susceptibility testing
Autores: | d'Azevedo,Pedro A.; Gonçalves,Ana Lúcia S.; Musskopf,Maiara I.; Ramos,Carina G.; Dias,Cícero A. G.
Data: | 2004-10-01
Ano: | 2004
Palavras-chave: | ESBL; Double disk confirmatory test; Cefoxitin susceptibility testing; Screening test.
Resumo: | Extended spectrum beta-lactamase (ESBL) production by Klebsiella sp. and E. coli is an emerging problem. In this study, 107 clinical isolates (53 E. coli, 47 K. pneumoniae and 7 K. oxytoca) screened as ESBL producers by the NCCLS disk diffusion procedure were submitted to a double disk confirmatory test (DDT) and to the E-test double strip for confirmation of ESBL production by demonstration of clavulanic acid inhibition effect (CAIE). Only 72/107 (67%) of the isolates were confirmed as ESBL producers by DDT, with diverse results among species. By the E-test, 58/107 (54%) isolates were confirmed as ESBL producers, and 18/107 (17%) were not determinable. Susceptibility to cefoxitin was found in 57/68 (83%) of strains that did not show CAIE. ESBL detection remains a controversial issue and clinical laboratories are in need of a simple and effective way to recognize strains with this kind of resistance.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000500006
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1590/S1413-86702004000500006
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.8 n.5 2004
Fonte: | info:eu-repo/semantics/openAccess