Data/hora: 10/06/2024 14:08:11

Provedor de dados: 	Brazilian Journal of Infectious Diseases	
País: 	Brazil	
Título: 	Laboratory tests in the detection of extended spectrum beta-lactamase production: National Committee for Clinical Laboratory Standards (NCCLS) screening test, the E-test, the double disk confirmatory test, and cefoxitin susceptibility testing	
Autores: 	d'Azevedo,Pedro A.; Gonçalves,Ana Lúcia S.; Musskopf,Maiara I.; Ramos,Carina G.; Dias,Cícero A. G.	
Data: 	2004-10-01	
Ano: 	2004	
Palavras-chave: 	ESBL; Double disk confirmatory test; Cefoxitin susceptibility testing; Screening test.	
Resumo: 	Extended spectrum beta-lactamase (ESBL) production by Klebsiella sp. and E. coli is an emerging problem. In this study, 107 clinical isolates (53 E. coli, 47 K. pneumoniae and 7 K. oxytoca) screened as ESBL producers by the NCCLS disk diffusion procedure were submitted to a double disk confirmatory test (DDT) and to the E-test double strip for confirmation of ESBL production by demonstration of clavulanic acid inhibition effect (CAIE). Only 72/107 (67%) of the isolates were confirmed as ESBL producers by DDT, with diverse results among species. By the E-test, 58/107 (54%) isolates were confirmed as ESBL producers, and 18/107 (17%) were not determinable. Susceptibility to cefoxitin was found in 57/68 (83%) of strains that did not show CAIE. ESBL detection remains a controversial issue and clinical laboratories are in need of a simple and effective way to recognize strains with this kind of resistance.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000500006	
Editor: 	Brazilian Society of Infectious Diseases	
Relação: 	10.1590/S1413-86702004000500006	
Formato: 	text/html	
Fonte: 	Brazilian Journal of Infectious Diseases  v.8 n.5 2004	
Fonte: 	info:eu-repo/semantics/openAccess