Data/hora: 09/06/2024 17:23:30

Provedor de dados: | Brazilian Journal of Infectious Diseases
País: | Brazil
Título: | Epstein-Barr virus DNA load and its association with Helicobacter pylori infection in gastroduodenal diseases
Autores: | Shukla,Sanket Kumar; Prasad,K.N; Tripathi,Aparna; Singh,Avinash; Saxena,Ashish; Ghoshal,Uday Chand; Krishnani,Narendra; Husain,Nuzhat.
Data: | 2011-12-01
Ano: | 2011
Palavras-chave: | Epstein-Barr virus infections; Stomach neoplasms; Helicobacter pylori.
Resumo: | Helicobacter pylori and Epstein-Barr virus (EBV) infections are common worldwide. Although H. pylori infection is a major factor in gastroduodenal diseases, its role in association with EBV infection is unknown. Objective: To study the association of H. pylori infection and EBV DNA load in patients with gastroduodenal diseases. Methods: Biopsy samples were collected from 200 adult patients [non-ulcer dyspepsia (NUD) 100, peptic ulcer disease (PUD) 50, gastric carcinoma (GC) 50] undergoing upper gastrointestinal endoscopy. H. pylori infection was diagnosed by rapid urease test, culture, histopathology, PCR and Q-PCR. EBV DNA was detected by non-polymorphic Epstein-Barr nuclear antigen-1 (EBNA-1) gene based Q-PCR. Results: In patients with GC and PUD, EBV DNA was detected more often than NUD (GC versus NUD = 90% versus 37%, p &lt; 0.001; PUD versus NUD = 70% versus 37%, p &lt; 0.001). The dual prevalence of H. pylori infection and EBV DNA was significantly higher in patients with GC and PUD than in those with NUD. Median copy number of EBV DNA was considerably higher in GC and PUD than NUD (p &lt; 0.01). The copy number of EBV DNA was significantly higher in H. pylori infected patients (p = 0.015). The number of ureA gene copies was also found to be significantly higher in PUD and NUD with presence of EBV DNA. However, in GC no significant difference was seen between EBV positive and negative status. Conclusion: There was a trend for higher EBV DNA load in H. pylori positive individuals suggesting a probable role of H. pylori in modulating the conversion of EBV to its lytic phase.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000600014
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1590/S1413-86702011000600014
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.15 n.6 2011
Fonte: | info:eu-repo/semantics/openAccess