Data/hora: 11/06/2024 11:09:25

Provedor de dados: | 55
País: | Brazil
Título: | Human papillomavirus detection and typing using a nested-PCR-RFLP assay
Autores: | Coser,Janaina; Boeira,Thaís da Rocha; Fonseca,André Salvador Kazantzi; Ikuta,Nilo; Lunge,Vagner Ricardo.
Data: | 2011-10-01
Ano: | 2011
Palavras-chave: | DNA probes; HPV polymerase chain reaction polymorphism; Restriction fragment length.
Resumo: | BACKGROUND: It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. OBJECTIVES: Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to detect and type HPV based on the analysis of L1 gene. METHODS: Analysis of published DNA sequence of mucosal HPV types to select sequences of new primers. Design of an original nested-PCR assay using the new primers pair selected and classical MY09/11 primers. HPV detection and typing in cervical samples using the nested-PCR-RFLP assay. RESULTS: The nested-PCR-RFLP assay detected and typed HPV in cervical samples. Of the total of 128 clinical samples submitted to simple PCR and nested-PCR for detection of HPV, 37 (28.9%) were positive for the virus by both methods and 25 samples were positive only by nested-PCR (67.5% increase in detection rate compared with single PCR). All HPV positive samples were effectively typed by RFLP assay. CONCLUSION: The method of nested-PCR proved to be an effective diagnostic tool for HPV detection and typing.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000500009
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1590/S1413-86702011000500009
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.15 n.5 2011
Fonte: | info:eu-repo/semantics/openAccess