Data/hora: 09/06/2024 12:31:41

Provedor de dados: | 55
País: | Brazil
Título: | Spontaneous neutrophil activation in HTLV-1 infected patients
Autores: | Guerreiro,Jaqueline B.; Porto,Maria Aurélia F.; Santos,Silvane B.; Lacerda,Lino; Ho,John L.; Carvalho,Edgar M.
Data: | 2005-12-01
Ano: | 2005
Palavras-chave: | HTLV-1; IFN-gamma; TNF-alpha; LPS; Neutrophil activation.
Resumo: | Human T cell lymphotropic Virus type-1 (HTLV-1) induces lymphocyte activation and proliferation, but little is known about the innate immune response due to HTLV-1 infection. We evaluated the percentage of neutrophils that metabolize Nitroblue tetrazolium (NBT) to formazan in HTLV-1 infected subjects and the association between neutrophil activation and IFN-gamma and TNF-alpha levels. Blood was collected from 35 HTLV-1 carriers, from 8 patients with HAM/TSP (HTLV-1- associated myelopathy); 22 healthy individuals were evaluated for spontaneous and lipopolysaccharide (LPS)-stimulated neutrophil activity (reduction of NBT to formazan). The production of IFN-gamma and TNF-alpha by unstimulated mononuclear cells was determined by ELISA. Spontaneous NBT levels, as well as spontaneous IFN-gamma and TNF-alpha production, were significantly higher (p&lt;0.001) in HTLV-1 infected subjects than in healthy individuals. A trend towards a positive correlation was noted, with increasing percentage of NBT positive neutrophils and levels of IFN-gamma. The high IFN-gamma producing HTLV-1 patient group had significantly greater NBT than healthy controls, 43±24% and 17±4.8% respectively (p&lt; 0.001), while no significant difference was observed between healthy controls and the low IFN-gamma-producing HTLV-1 patient group (30±20%). Spontaneous neutrophil activation is another marker of immune perturbation resulting from HTLV-1 infection. In vivo activation of neutrophils observed in HTLV-1 infected subjects is likely to be the same process that causes spontaneous IFN-gamma production, or it may partially result from direct IFN-gamma stimulation.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000600010
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1590/S1413-86702005000600010
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.9 n.6 2005
Fonte: | info:eu-repo/semantics/openAccess