Data/hora: 08/06/2024 17:22:48

Provedor de dados: | Brazilian Journal of Infectious Diseases
País: | Brazil
Título: | Detection of hepatitis B virus A1762T/G1764A mutant by amplification refractory mutation system
Autores: | Wang,Yong-Zhong; Zhu,Zhen; Zhang,Hong-Yu; Zhu,Min-Zhi; Xu,Xin; Chen,Chun-Hua; Liu,Long-Gen.
Data: | 2014-06-01
Ano: | 2014
Palavras-chave: | Hepatitis B virus; Basal core promoter mutations; Amplification refractory mutation system; PCR.
Resumo: | OBJECTIVE: To study the role of hepatitis B virus with A1762T/G1764A double mutation in liver cirrhosis and hepatocellular carcinoma, and create a sensitive, fast, accurate assay for detection of A1762T/G1764A double mutation.METHODS: We developed an accurate and fast real-time amplification refractory mutation system to detect A1762T/G1764A double mutation. Cloned hepatitis B virus genome was used as a control. Assay sensitivity was determined by serial dilution and mixed template experiments. Specificity was determined by cross experiments with wild and mutant hepatitis B virus. Fifty clinical samples were tested by the real-time amplification refractory mutation system and the results were compared with sequencing.RESULTS: The real-time amplification refractory mutation system had a sensitivity of 100 copies of virus with these mutations, and 0.1% weak population virus with double mutation could be found in mixtures. A total of 50 randomly collected clinical samples were detected by real-time amplification refractory mutation system, and the results were consistent with those by DNA sequencing. Hepatitis B virus genotype C was more prevalent in 39 of 50 samples than genotype B (11 samples), and about 75% of genotype C carried a double mutation compared to 45% of genotype B. However, the percentage of A1762T/G1764A double mutation in hepatitis B e antigen-negative (58.3%) samples was almost the same as in hepatitis B e antigen-positive (61%) samples.CONCLUSION: The real-time amplification refractory mutation system is sensitive and specific for detection of hepatitis B virus double mutation.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300261
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1016/j.bjid.2013.09.005
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.18 n.3 2014
Fonte: | info:eu-repo/semantics/openAccess