Data/hora: 10/06/2024 08:18:55

Provedor de dados: | 55
País: | Brazil
Título: | Interleukin-12 gene adjuvant increases the immunogenicity of virus-like particles of human papillomavirus type 16 regional variant strain
Autores: | Wei,Lanlan; Chu,Ming; Zhang,Qingmeng; Wang,Yan; Shang,Qinglong; Zhang,Yunyan; Zhang,Guangmei.
Data: | 2014-01-01
Ano: | 2014
Palavras-chave: | Cervical cancer; Human papillomavirus type 16; Virus-like particle; Cellular immunity; IL-12.
Resumo: | Objectives:To analyze the immunogenicity of virus-like particles (VLP) of human papillomavirus type 16 (HPV16) isolated in East China and the adjuvant potential of interleukin-12 (IL-12).Methods:The variant HPV16 L1VLP expressed in sf9 insect cells were purified with cesium chloride gradient centrifugation. BALB/c mice were vaccinated with VLP (L1N), VLP with Freund's adjuvant (L1A) or VLP with IL-12 recombinant plasmid (L1P). HPV16 VLP specific IgG and IFN-γ level in the serum were detected by ELISA, and the percentage of CD4+ and CD8+ in spleen cells was detected with flow cytometry.Results:The titers of serum IgG antibodies in vaccinated groups were higher than in negative control and the serum antibodies mainly recognized conformation-dependent HPV16 VLP epitopes. Splenic CD4+ and CD8+ T cell subsets increased after vaccination in every experimental group, and CD8+ increased obviously in L1P group. The ratio of CD4+/CD8+ decreased in L1P group and increased in the other two groups, compared to control group. Vaccination induced specific secretion of IFN-γ in the serum of vaccinated group (p &lt; 0.05), especially in the L1P group.Conclusions:VLP of HPV16 variant strain isolated in East China could induce humoral immunity and cellular immunity in mice, and IL-12 recombinant plasmid can enhance cellular immunity.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000100065
Editor: | Brazilian Society of Infectious Diseases
Relação: | 10.1016/j.bjid.2013.05.015
Formato: | text/html
Fonte: | Brazilian Journal of Infectious Diseases  v.18 n.1 2014
Fonte: | info:eu-repo/semantics/openAccess